THE DETERMINATION OF GLYCINE IN PROTEIN HYDROLYSATES WITH LEUCONOSTOC MESENTEROIDES P-60

Media for Leuconostoc mesenteroides P-60 and Leuconostoc citrovorum 8081.

In the routine use of typical media for the microbiological determination of the sixteen amino acids standard curves have accumulated which reveal the amounts of each amino acid required for the growth of the assay organism when all other amino acids are present in excess. Thus it was observed that the relative amounts of the various amino acids needed by Leuconostoc mesenteroides P-60 did not ...

Investigations of amino acids, peptides, and proteins; the determination of phenylalanine in protein hydrolysates with Leuconostoc mesenteroides P-60 and Lactobacillus casei.

During the past 2 years microbiological procedures’ have been reported for the determination in proteins of thirteen of the twenty-one amino acids which occur in protein materials. Seven amino acids (isoleucine, glutamic acid, methionine, leucine, phenylalanine, tryptophane, and valine) have been determined with Lactobacillus arabinosus 17-5, three (arginine, tyrosine, and valine) with Lactobac...

The micro-determination of glycine in protein hydrolysates.

The response of Leuconostoc mesenteroides P-60 to some compounds related to lysine.

The bacterium, Leuconostoc mesenteroides P-60, is commonly used in the assay of lysine (1, 2). In view of this fact, it is useful to know how specific or selective the organism is for this purpose. It is known that some organisms can dispense with lysine if related metabolites are provided. For example, both a-aminoadipic and oc-amino-e-hydroxycaproic acids satisfy the lysine requirements of so...

An Improved Microbiological Method for the De- Termination of Cystine in Human Urine with Leuconostoc Mesenteroides P-60*

Investigations concerning the urinary excretion of cystine by normal subjects and by individuals with cystinuria, liver cirrhosis, diabetes, tuberculosis, and other pathological conditions have been reported previously from this laboratory (2-6). In continuing these studies, modification of the cystine assay method seemed desirable to minimize destruction of cystine which may occur in the mediu...